Volume 6, Issue 5, September 2018, Page: 126-131
A Comparison of One Shape and ProTaper Next Rotary Systems for Elimination of Enterococcus Faecalis from Root Canal: Microbiological and SEM Evaluation
Renu Bala Sroa, Department of Conservative Dentistry and Endodontics, Punjab Government Dental College & Hospital, Amritsar, India
Baljeet Sidhu, Department of Conservative Dentistry and Endodontics, Punjab Government Dental College & Hospital, Amritsar, India
Sangeeta Aggarwal, Department of Conservative Dentistry and Endodontics, Punjab Government Dental College & Hospital, Amritsar, India
Aakanksha Chopra, Department of Conservative Dentistry and Endodontics, Punjab Government Dental College & Hospital, Amritsar, India
Angelina Bedi, Department of Conservative Dentistry and Endodontics, Punjab Government Dental College & Hospital, Amritsar, India
Received: Jun. 13, 2018;       Accepted: Jul. 24, 2018;       Published: Sep. 17, 2018
DOI: 10.11648/j.ajim.20180605.16      View  171      Downloads  20
Abstract
Incomplete removal of micro- organisms from infected root canals is a common cause of failed endodontic treatment. The difficulty in eradication of Enterococcus faecalis from root canals plays an essential role in pathogenesis of persistent pulpal and periradicular infections. The aim of the present study was to compare the reduction of Enterococcus faecalis in root canals by mechanical instrumentation using two rotary systems (One Shape and ProTaper Next) and Hand K-file instrumentation by using microbiological and Scanning Electron Microscopy (SEM) evaluation. Fifty one freshly extracted mandibular premolars with a single root were collected. After pre- instrumentation sampling, they were divided into three groups, Group A, Group B and Group C in which biomechanical preparation was done using Hand K- File, OneShape and Protaper Next respectively. Reduction in pre- instrumentation and post- instrumentation values of Enterococcus faecalis were analysed using microbiological and SEM evaluation. Statistical analysis by paired ‘t’ test and p value showed that there was highly statistical significant difference in CFU count reduction between the pre-instrumentation and post-instrumentation values in all the groups (p<0.001). Statistical analysis by Kruskal-Wallis Test and Mann-Whitney Test showed that at 1mm and 3mm level, Group A (Hand K-File) scored significantly higher value followed by Group B (OneShape Apical) and Group C (ProTaper Next). The most effective instrumentation technique in eliminating Enterococcus faecalis from the root canal was ProTaper Next system in comparison to OneShape Apical and Hand K-File.
Keywords
Enterococcus Faecalis, Hand K-File, OneShape Apical, ProTaper Next
To cite this article
Renu Bala Sroa, Baljeet Sidhu, Sangeeta Aggarwal, Aakanksha Chopra, Angelina Bedi, A Comparison of One Shape and ProTaper Next Rotary Systems for Elimination of Enterococcus Faecalis from Root Canal: Microbiological and SEM Evaluation, American Journal of Internal Medicine. Vol. 6, No. 5, 2018, pp. 126-131. doi: 10.11648/j.ajim.20180605.16
Copyright
Copyright © 2018 Authors retain the copyright of this article.
This article is an open access article distributed under the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Reference
[1]
Pinheiro S L, Pessoa C, Silva J N, Gonçalves R O, Duarte D A, Bueno C E S. Comparative Analysis of Protaper and Waveone Systems to Reduce Enterococcus Faecalis from Root Canal System in Primary Molars –An in Vitro Study. J Clin Pediatr Dent 2016; 40: 124-128.
[2]
Bystrom A, Sundqvist G. Bacteriologic evaluation of the efficacy of mechanical instrumentation in endodontic therapy. Scand J Dent Res 1981; 89: 321–328.
[3]
Foschi F, Nucci C, Montebugnoli L. SEM evaluation of canal wall dentin following use of Mtwo and ProTaper NiTi rotary instruments. Int Endod J 2004; 37: 832-839.
[4]
[urklein S, Benten S, Schafer E. Quantitative evaluation of apically extruded debris with different single-file systems: Reciproc, F360 and OneShape versus Mtwo. Int Endod J 2014; 47: 405–409.
[5]
Mittal R, Singla M G, Garg A, Dhawan A. A Comparison of Apical Bacterial Extrusion in Manual, ProTaper Rotary, and One Shape Rotary Instrumentation Techniques. J Endod 2015; 41: 2040–2044.
[6]
[handrasekhar P, Shetty R U, Adlakha T, Shende S, Podar R. A comparison of two NiTi rotary systems, ProTaper Next and Silk for root canal cleaning ability (An in vitro study). Ind J Conserv Endod 2016; 1: 22-24.
[7]
Varghese N O, Pillai R, Sujathen U, Sainudeen S, Antony A, Paul S. Resistance to torsional failure and cyclic fatigue resistance of ProTaper Next, WaveOne, and Mtwo files in continuous and reciprocating motion: An in vitro study. J Conserv Dent 2016; 19: 225-230.
[8]
Hartroth, B, Seyfahrt I, Conrads G. Sampling of perìodontal pathogens by paper points: evaluation of basic parameters. Oral Microbiology and Immunology 1999; 14: 326-330.
[9]
Paranjpe A, Gregorio C, Gonzalez A M, Gomez A, Herzog D S, Pina A A, MS, Cohenca N. Efficacy of the Self-Adjusting File System on Cleaning and Shaping Oval Canals: A Microbiological and Microscopic Evaluation. J Endod 2012; 38: 226–231.
[10]
[skandarinezhad M, Sadrhaghighi1 H, Rahmani M, Majd N M, Sadighi A. Comparison of two mechanical instrumentation techniques in reducing root canal bacterial population. Afr J Microbiol Res 2012; 6: 3635-3638.
[11]
[ydin C, Tunca Y M, Senses Z, Baysallar M, Kayaoglu G, Orstavik D. Bacterial reduction by extensive versus conservative root canal instrumentation in vitro. Acta Odontol Scand 2007; 65: 167-170.
[12]
Vossoghi M, Vossoghi M, Shahriari S, Faramarzi F, Mashouf R Y, Farhadian M. Efficacy of a Novel Rotary System in Reduction of Intracanal Bacteria: An in Vitro Study. Iran Endod J 2016; 11: 219-222.
[13]
Karatas E, Gultekin E, Arslan H, Kirici D O, Alsancak M, Topcu M C. Evaluation of instrumentation systems in reducing E. faecalis from root canals: TF adaptive and ProTaper next versus single file systems. Int J Artif Organs. 2015; 38: 161-164.
[14]
[abeshima C K, Caballero-Flores H, Cai S, Aranguren J, Britto M L B, Machado M E L. Bacterial Removal Promoted by 2 Single-file Systems: Wave One and One Shape. J Endod 2014; 40: 1995–1998.
[15]
Demiryurek E O, Kalyoncuoglu E, Duran E, Coban A Y, Cayc Y T. Efficacy of different instrumentation techniques on reducing Enterococcus faecalis infection in experimentally infected root canals. J Dent Sci 2014; 9: 23-28.
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